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CRTAC1 Monoclonal antibody

CRTAC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse, rabbit, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11E2

Cat no : 68276-1-Ig

Synonyms

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAC1 Monoclonal antibody (68276-1-Ig)

    WB analysis using 68276-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68276-1-Ig (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRTAC1 Monoclonal antibody (68276-1-Ig)

    IF Staining of HeLa using 68276-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAC1 antibody (68276-1-Ig, Clone: 2A11E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, rabbit cerebellum tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68276-1-Ig targets CRTAC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.

Tested Reactivity Human, rat, mouse, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CRTAC1 fusion protein Ag32813
Full Name cartilage acidic protein 1
Calculated Molecular Weight 661 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC034245
Gene Symbol CRTAC1
Gene ID (NCBI) 55118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTAC1 (cartilage acidic protein 1) is also named as ASPIC1 and CEP68. CRTAC1 is an extracellular matrix protein of human chondrogenic tissue. CRTAC1 promotes cell proliferation, migration and extracellular matrix regeneration and remodeling in primary human fibroblasts (PMID:32084494). CRTAC1 expression is decreased in tissue samples of bladder urothelial carcinoma (PMID:34818994). CRTAC1 overexpression promotes the pyroptosis of human lens epithelial cells by facilitating the reactive oxygen species (ROS) production in the formation of cataract (PMID:32838966). CRTAC1 was the most compelling and robust biomarker for osteoarthritis severity and progression (PMID:35924962).

Protocols

Product Specific Protocols
WB protocol for CRTAC1 antibody 68276-1-IgDownload protocol
IF protocol for CRTAC1 antibody 68276-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols