CoraLite® Plus 488-conjugated CRNKL1 Recombinant antibody

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  IF Staining of MCF-7 using CL488-83914

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 CRNKL1 antibody (CL488-83914, Clone: 240697D12 ) at dilution of 1:200.

CRNKL1 Recombinant Antibody for IF/ICC

Cat No. CL488-83914

Clone No.240697D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83914 targets CRNKL1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: crooked neck pre-mRNA splicing factor-like 1 (Drosophila)
• Calculated Molecular Weight: 100 kDa
• GenBank Accession Number: NM_016652
• Gene Symbol: CRNKL1
• Gene ID (NCBI): 51340
• RRID: AB_3673325
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BZJ0
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CRNKL1 (Crooked Neck Pre-MRNA Splicing Factor 1) is involved in pre-mRNA splicing process. As a component of the minor spliceosome, it's involved in the splicing of U12-type introns in pre-mRNAs. It's reported that CRNKL1 is a nuclear retention factor of the HIV-1 unspliced RNA and a selective regulator of cytoplasmic levels of a subset of intron-retaining cellular mRNAs.(PMID: 33468685)