Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP1 antibody (CL488-68021, Clone: 2F4G5 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP1 antibody (CL488-68021, Clone: 2F4G5 ) at dilution of 1:200.
FC experiment of U2OS using CL488-68021
1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CRMP1 (CL488-68021, Clone:2F4G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CRMP1 (CL488-68021, Clone:2F4G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Background Information
CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.
Protocols
Product Specific Protocols
IF protocol for CL Plus 488 CRMP1 antibody CL488-68021
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP1 antibody (CL488-68021, Clone: 2F4G5 ) at dilution of 1:200.
FC (INTRA) Figures
FC experiment of U2OS using CL488-68021
1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CRMP1 (CL488-68021, Clone:2F4G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CRMP1 Monoclonal antibody
Catalog Number
CL488-68021
Citations
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Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
human, mouse, rat, pig, rabbit, chicken
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
