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CoraLite® Plus 488-conjugated CRMP1 Monoclonal antibody

CRMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F4G5

Cat no : CL488-68021

Synonyms

DRP 1, DPYSL1, Dihydropyrimidinase-related protein 1, CRMP-1, CRMP 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated CRMP1 Monoclonal ant (CL488-68021)

    IF Staining of SH-SY5Y using CL488-68021

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP1 antibody (CL488-68021, Clone: 2F4G5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated CRMP1 Monoclonal ant (CL488-68021)

    FC experiment of U2OS using CL488-68021

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CRMP1 (CL488-68021, Clone:2F4G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68021 targets CRMP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CRMP1 fusion protein Ag30090
Full Name collapsin response mediator protein 1
Calculated Molecular Weight 62 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC000252
Gene Symbol CRMP1
Gene ID (NCBI) 1400
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CRMP1 antibody CL488-68021Download protocol
Standard Protocols
Click here to view our Standard Protocols