CRHR2 Polyclonal antibody, PBS Only

• 

  WB analysis of rat skeletal muscle using 25267-1-AP (same clone as 25267-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 25267-1-AP (same clone as 25267-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25267-1-AP (same clone as 25267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25267-1-AP (same clone as 25267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25267-1-PBS in a different storage buffer formulation.

CRHR2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 25267-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2, CRF R 2, CRF R2, CRF2R

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Product Information

25267-1-PBS targets CRHR2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: corticotropin releasing hormone receptor 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_001883
• Gene Symbol: CRHR2
• Gene ID (NCBI): 1395
• RRID: AB_2879996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13324
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CRHR2 is a receptor for corticotropin-releasing factor (CRF) and belongs to the G-protein coupled receptor family. It shows high affinity for CRF and also binds to related peptides such as urocortin I, II, and III. This receptor is involved in mediating the endocrine, autonomic, and behavioral responses to stress and immune challenges.