CRHR2 Polyclonal antibody

CRHR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25267-1-AP

Synonyms

CRF R 2, CRF R2, CRF2R, CRFR 2, CRFR2, CRH R 2, CRH R2, CRH2R, CRHR2

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Validation Data Gallery

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  WB analysis of rat skeletal muscle using 25267-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25267-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, mouse heart tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

25267-1-AP targets CRHR2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: corticotropin releasing hormone receptor 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_001883
• Gene Symbol: CRHR2
• Gene ID (NCBI): 1395
• RRID: AB_2879996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Pharmaceuticals (Basel); A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors; Authors - Yueshan Sun
mouse	WB	Cell Signal; Activation of CRHR1 contributes to cerebral endothelial barrier impairment via cPLA2 phosphorylation in experimental ischemic stroke.; Authors - Changchun Cao
human,rat	IHC,WB	Neurogastroenterol Motil; Lidocaine ameliorates intestinal barrier dysfunction in irritable bowel syndrome by modulating corticotropin-releasing hormone receptor 2; Authors - Yanrong Wang
mouse	IF	Reprod Sci; Melatonin Protects Against Mitochondrial Dyshomeostasis and Ovarian Damage Caused by Chronic Unpredictable Mild Stress Through the eIF2α-AFT4 Signaling Pathway in Mice; Authors - Si-Min Ding