CREST Monoclonal antibody, PBS Only
CREST Monoclonal Antibody for WB, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse
Applications
WB, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
3D5D10
Cat no : 60314-1-PBS
Synonyms
Validation Data Gallery
Product Information
60314-1-PBS targets CREST in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CREST fusion protein Ag3119 |
Full Name | synovial sarcoma translocation gene on chromosome 18-like 1 |
Calculated Molecular Weight | 396 aa, 43 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC034494 |
Gene Symbol | CREST |
Gene ID (NCBI) | 26039 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).