CPT2 Polyclonal antibody

CPT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

40

Reviews (1)

Cat no : 26555-1-AP

Synonyms

EC:2.3.1.21, CPTASE, CPT II, carnitine palmitoyltransferase 2, Carnitine O-palmitoyltransferase 2, mitochondrial

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Validation Data Gallery

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26555-1-AP

  IP result of anti-CPT2 (IP:26555-1-AP, 4ug; Detection:26555-1-AP 1:300) with mouse liver tissue lysate 6000ug.

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  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 26555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 26555-1-AP (CPT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, T-47D cells, LNCaP cells, mouse liver tissue, rat liver tissue, MCF-7 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 36 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 6 publications below

Product Information

26555-1-AP targets CPT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPT2 fusion protein Ag24897
• Full Name: carnitine palmitoyltransferase 2
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002445
• Gene Symbol: CPT2
• Gene ID (NCBI): 1376
• RRID: AB_2880551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
rat	WB,IP	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
mouse	WB	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li
mouse	WB,IP	Elife; Remodeling of whole-body lipid metabolism and a diabetic-like phenotype caused by loss of CDK1 and hepatocyte division.; Authors - Jin Rong Ow
mouse	WB	J Nanobiotechnology; Regulating immune memory and reversing tumor thermotolerance through a step-by-step starving-photothermal therapy; Authors - Lihua Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mitchell (Verified Customer) (09-26-2019)

Worked great for western blot in proximal intestine and liver.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Intestine and Liver