CPT2 Polyclonal antibody

CPT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (1)

Cat no : 26555-1-AP

Synonyms

CPT II, CPT1, CPT2, CPTASE

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Validation Data Gallery

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26555-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 26555-1-AP

  IP result of anti-CPT2 (IP:26555-1-AP, 4ug; Detection:26555-1-AP 1:300) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 26555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 26555-1-AP (CPT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse heart tissue, T-47D cells, MCF-7 cells, LNCaP cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 24 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Product Information

26555-1-AP targets CPT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPT2 fusion protein Ag24897
• Full Name: carnitine palmitoyltransferase 2
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002445
• Gene Symbol: CPT2
• Gene ID (NCBI): 1376
• RRID: AB_2880551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
rat	WB,IP	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
mouse	WB	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li
mouse	WB,IP	Elife; Remodeling of whole-body lipid metabolism and a diabetic-like phenotype caused by loss of CDK1 and hepatocyte division.; Authors - Jin Rong Ow
mouse	WB	J Nanobiotechnology; Regulating immune memory and reversing tumor thermotolerance through a step-by-step starving-photothermal therapy; Authors - Lihua Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mitchell (Verified Customer) (09-26-2019)

Worked great for western blot in proximal intestine and liver.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Intestine and Liver