Filter: AllWBIHC

WB analysis using 66039-3-Ig (same clone as 66039-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 66039-3-Ig (same clone as 66039-3-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
WB analysis using 66039-3-Ig (same clone as 66039-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
WB analysis using 66039-3-Ig (same clone as 66039-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66039-3-Ig (same clone as 66039-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66039-3-Ig (same clone as 66039-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
IHC staining of rat heart using 66039-3-Ig (same clone as 66039-3-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
IHC staining of rat heart using 66039-3-Ig (same clone as 66039-3-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 66039-3-Ig (same clone as 66039-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 66039-3-Ig (same clone as 66039-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-3-PBS in a different storage buffer formulation.

CPT1A Monoclonal antibody, PBS Only

CPT1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66039-3-PBS

Clone No.5D2B8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine O-palmitoyltransferase 1, liver isoform

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66039-3-PBS targets CPT1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CPT1A fusion protein Ag7745 Predict reactive species
Full Name	carnitine palmitoyltransferase 1A (liver)
Calculated Molecular Weight	88 kDa
Observed Molecular Weight	86 kDa
GenBank Accession Number	BC000185
Gene Symbol	CPT1A
Gene ID (NCBI)	1374
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P50416
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.