Filter: AllWBIHC

WB analysis using 66039-3-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 66039-3-Ig
mouse liver tissue were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 66039-3-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 66039-3-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 66039-3-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 66039-3-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 66039-3-Ig
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 66039-3-Ig
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 66039-3-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 66039-3-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CPT1A Monoclonal antibody

CPT1A Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66039-3-Ig

Clone No.5D2B8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

5D2B8, Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	TT cells, HeLa cells, rat liver tissue, mouse liver tissue, LNCaP cells, PANC-1 cells, HaCaT cells
Positive IHC detected in	mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66039-3-Ig targets CPT1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CPT1A fusion protein Ag7745 Predict reactive species
Full Name	carnitine palmitoyltransferase 1A (liver)
Calculated Molecular Weight	88 kDa
Observed Molecular Weight	86 kDa
GenBank Accession Number	BC000185
Gene Symbol	CPT1A
Gene ID (NCBI)	1374
RRID	AB_3670374
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P50416
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.