CPS1 Recombinant antibody
CPS1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241198F1
Cat no : 84277-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse liver tissue, rat liver tissue |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84277-1-RR targets CPS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | carbamoyl-phosphate synthetase 1, mitochondrial |
Calculated Molecular Weight | 165 kDa |
Observed Molecular Weight | 140-165 kDa |
GenBank Accession Number | BC140943 |
Gene Symbol | CPS1 |
Gene ID (NCBI) | 1373 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for CPS1 antibody 84277-1-RR | Download protocol |
IF protocol for CPS1 antibody 84277-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |