CPS1 Monoclonal antibody

CPS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12A4

Cat no : 67630-1-Ig

Synonyms

CPS1, CPSase I

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Validation Data Gallery

• 

  WB analysis of mouse liver using 67630-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67630-1-Ig (CPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67630-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67630-1-Ig (CPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67630-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67630-1-Ig (CPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67630-1-Ig targets CPS1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: carbamoyl-phosphate synthetase 1, mitochondrial
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 140-165 kDa
• GenBank Accession Number: BC140943
• Gene Symbol: CPS1
• Gene ID (NCBI): 1373
• RRID: AB_2882831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine.