COX5B Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

COX5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11418-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

  WB result of COX5B antibody (11418-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX5B transfected HeLa cells. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  WB analysis using 11418-2-AP (same clone as 11418-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11418-2-AP (COX5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11418-2-AP (same clone as 11418-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11418-2-AP (same clone as 11418-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11418-2-AP (COX5B antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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Product Information

11418-2-PBS targets COX5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX5B fusion protein Ag1986
• Full Name: cytochrome c oxidase subunit Vb
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC006229
• Gene Symbol: COX5B
• Gene ID (NCBI): 1329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)