|Positive WB detected in
|HeLa cells, L02 cells, mouse liver tissue, rat liver tissue
|Positive IHC detected in
|human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:250-1:1000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11418-2-AP targets COX5B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, goat, monkey
|Host / Isotype
|Rabbit / IgG
|COX5B fusion protein Ag1986
|cytochrome c oxidase subunit Vb
|Calculated molecular weight
|129 aa, 14 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)
A cytosolic surveillance mechanism activates the mitochondrial UPR
Mechanism of super-assembly of respiratory complexes III and IV.
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense.
Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.
Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Süleyman (Verified Customer) (02-07-2022)
HeLa cell line is used and even though it has some unspecific bands but it is very specific and the unspecific bands are very distant. Recommended!