CORO1A Recombinant antibody, PBS Only

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  WB analysis using 84602-5-RR (same clone as 84602-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84602-5-RR (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CORO1A antibody (84602-5-RR, Clone: 242027H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CORO1A Recombinant antibody (84602-5-RR, Clone:242027H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

CORO1A {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84602-5-PBS

Clone No.242027H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CORO1, Clipin-A, CLIPINA, Clipin A, CLABP

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Product Information

84602-5-PBS targets CORO1A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CORO1A fusion protein Ag12139
• Full Name: coronin, actin binding protein, 1A
• Calculated Molecular Weight: 461 aa, 51 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC110374
• Gene Symbol: CORO1A
• Gene ID (NCBI): 11151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31146
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).