CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17760-1-AP

Synonyms

CLABP, Clipin A, CLIPINA, CORO1, CORO1A, Coronin 1A, Coronin like protein A, Coronin like protein p57, HCORO1, p57, TACO

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Validation Data Gallery

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  WB analysis of rat brain using 17760-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17760-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17760-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17760-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17760-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 17760-1-AP

  IP Result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.

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  IF Staining of mouse spleen using 17760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 17760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 17760-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CORO1A (17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat brain tissue, Jurkat cells, HL-60 cells, rat spleen tissue, mouse spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IF detected in: mouse spleen tissue, mouse colon tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

17760-1-AP targets CORO1A in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CORO1A fusion protein Ag12139
• Full Name: coronin, actin binding protein, 1A
• Calculated molecular weight: 461 aa, 51 kDa
• Observed molecular weight: 57 kDa
• GenBank accession number: BC110374
• Gene symbol: CORO1A
• Gene ID (NCBI): 11151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Br J Pharmacol; PMID: 27128203; Authors - Xianrong Zhang
human	WB	Int Immunopharmacol; Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.; Authors - Fuli Min
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song
rat	WB	PLoS One; Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.; Authors - Feng Guo