CoraLite® Plus 488-conjugated COPA Recombinant antibody

COPA Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230361G2

Cat no : CL488-83089

Synonyms

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

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Validation Data Gallery

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  IF Staining of A549 using CL488-83089

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 COPA antibody (CL488-83089, Clone: 230361G2 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83089

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 COPA Recombinant Antibody (CL488-83089, Clone:230361G2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83089 targets COPA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COPA fusion protein Ag34207
• Full Name: coatomer protein complex, subunit alpha
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_004371
• Gene Symbol: COPA
• Gene ID (NCBI): 1314
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.