COPA Recombinant antibody, PBS Only

COPA Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230361B1

Cat no : 83089-2-PBS

Synonyms

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

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Validation Data Gallery

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  WB analysis using 83089-2-RR (same clone as 83089-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83089-2-RR (COPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83089-2-RR (same clone as 83089-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83089-2-RR (COPA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83089-2-RR (same clone as 83089-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COPA antibody (83089-2-RR, Clone: 230361B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83089-2-RR (same clone as 83089-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COPA Recombinant antibody (83089-2-RR, Clone:230361B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83089-2-PBS is COPA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COPA fusion protein Ag34207
• Full Name: coatomer protein complex, subunit alpha
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_004371
• Gene Symbol: COPA
• Gene ID (NCBI): 1314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.