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COPA Recombinant antibody

COPA Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230361B1

Cat no : 83089-2-RR

Synonyms

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPA Recombinant antibody (83089-2-RR)

    WB analysis using 83089-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83089-2-RR (COPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COPA Recombinant antibody (83089-2-RR)

    IHC staining of human ovarian cancer using 83089-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83089-2-RR (COPA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPA Recombinant antibody (83089-2-RR)

    IF Staining of HeLa using 83089-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COPA antibody (83089-2-RR, Clone: 230361B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using COPA Recombinant antibody (83089-2-RR)

    FC experiment of HeLa using 83089-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COPA Recombinant antibody (83089-2-RR, Clone:230361B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HepG2, Jurkat, MCF-7, HT-29, NIH-3T3, C2C12
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83089-2-RR is COPA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPA fusion protein Ag34207
Full Name coatomer protein complex, subunit alpha
Calculated Molecular Weight138 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberNM_004371
Gene Symbol COPA
Gene ID (NCBI) 1314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.

Protocols

Product Specific Protocols
WB protocol for COPA antibody 83089-2-RRDownload protocol
IHC protocol for COPA antibody 83089-2-RRDownload protocol
IF protocol for COPA antibody 83089-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols