COMMD1 Monoclonal antibody, PBS Only

COMMD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B10E10

Cat no : 67016-1-PBS

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1

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Validation Data Gallery

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  WB analysis using 67016-1-Ig (same clone as 67016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67016-1-PBS targets COMMD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COMMD1 fusion protein Ag28596
• Full Name: copper metabolism (Murr1) domain containing 1
• Calculated Molecular Weight: 190 aa, 21 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC022046
• Gene Symbol: COMMD1
• Gene ID (NCBI): 150684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.