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COMMD1 Monoclonal antibody

COMMD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B10E10

Cat no : 67016-1-Ig

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COMMD1 Monoclonal antibody (67016-1-Ig)

    WB analysis using 67016-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COMMD1 Monoclonal antibody (67016-1-Ig)

    IHC staining of human breast cancer using 67016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COMMD1 Monoclonal antibody (67016-1-Ig)

    IHC staining of human breast cancer using 67016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COMMD1 Monoclonal antibody (67016-1-Ig)

    IF Staining of HepG2 using 67016-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHT-29 cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67016-1-Ig targets COMMD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMMD1 fusion protein Ag28596
Full Name copper metabolism (Murr1) domain containing 1
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC022046
Gene Symbol COMMD1
Gene ID (NCBI) 150684
RRIDAB_2882332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Protocols

Product Specific Protocols
WB protocol for COMMD1 antibody 67016-1-IgDownload protocol
IHC protocol for COMMD1 antibody 67016-1-IgDownload protocol
IF protocol for COMMD1 antibody 67016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols