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Collagen Type I Recombinant antibody, PBS Only

Collagen Type I Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240665F8

Cat no : 83752-5-PBS

Synonyms

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HSC-T6 cells using Collagen Type I Recombinant antibody (83752-5-RR)

    IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using COL1A2 antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HSC-T6 cells using Collagen Type I Recombinant antibody (83752-5-RR)

    IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using COL1A2 antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83752-5-RR

    Affinity and Kinetic Characterization of 83752-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-5-RR against Human COL1A2 were performed. The affinity constant is 6.77 nM.

Product Information

The immunogen of 83752-5-PBS is Collagen Type I Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281
Full Name collagen, type I, alpha 2
Calculated Molecular Weight 1366 aa, 130 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.