COASY Monoclonal antibody

COASY Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D1C11

Cat no : 68358-1-Ig

Synonyms

Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase

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Validation Data Gallery

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  WB analysis using 68358-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68358-1-Ig (COASY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human liver using 68358-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68358-1-Ig (COASY antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68358-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68358-1-Ig (COASY antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 68358-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug COASY Monoclonal antibody (68358-1-Ig, Clone:1D1C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68358-1-Ig targets COASY in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COASY fusion protein Ag33142
• Full Name: Coenzyme A synthase
• Calculated Molecular Weight: 564 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC020985
• Gene Symbol: COASY
• Gene ID (NCBI): 80347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.