CNTN1 Monoclonal antibody

CNTN1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1D4

Cat no : 68252-1-Ig

Synonyms

CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3

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Validation Data Gallery

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  WB analysis using 68252-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68252-1-Ig (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68252-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68252-1-Ig (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, pig cerebellum tissue, pig brain tissue, rabbit brain tissue, rabbit cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68252-1-Ig targets CNTN1 in WB, ELISA applications and shows reactivity with Human, pig, rabbit samples.

• Tested Reactivity: Human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNTN1 fusion protein Ag5030
• Full Name: contactin 1
• Calculated Molecular Weight: 1018 aa, 113 kDa
• Observed Molecular Weight: 130-135 kDa
• GenBank Accession Number: BC036569
• Gene Symbol: CNTN1
• Gene ID (NCBI): 1272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).