CNOT9 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CNOT9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22503-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

  WB result of RQCD1 antibody (22503-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RQCD1 transfected HepG2 cells. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  WB analysis using 22503-1-AP (same clone as 22503-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22503-1-AP (CNOT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 22503-1-AP (same clone as 22503-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22503-1-AP (RQCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 22503-1-AP (same clone as 22503-1-PBS)

  IP result of anti-CNOT9 (IP:22503-1-AP, 4ug; Detection:22503-1-AP 1:1000) with MCF-7 cells lysate 1280ug. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 22503-1-AP (same clone as 22503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 22503-1-AP (same clone as 22503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CNOT9 antibody (22503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 22503-1-AP (same clone as 22503-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CNOT9 antibody (22503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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Product Information

22503-1-PBS targets CNOT9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNOT9 fusion protein Ag18197
• Full Name: RCD1 required for cell differentiation1 homolog (S. pombe)
• Calculated Molecular Weight: 299 aa, 34 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC007102
• Gene Symbol: CNOT9
• Gene ID (NCBI): 9125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.