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- KD/KO Validated
CNOT9 Polyclonal antibody
CNOT9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 22503-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse thymus tissue, HepG2 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
22503-1-AP targets CNOT9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CNOT9 fusion protein Ag18197 |
| Full Name | RCD1 required for cell differentiation1 homolog (S. pombe) |
| Calculated Molecular Weight | 299 aa, 34 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC007102 |
| Gene Symbol | CNOT9 |
| Gene ID (NCBI) | 9125 |
| RRID | AB_11232413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| IHC protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| IF protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| IP protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation
| ||
Mol Cell Structural and Biochemical Insights to the Role of the CCR4-NOT Complex and DDX6 ATPase in MicroRNA Repression. | ||
Mol Cell A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing. | ||
Nat Commun RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.
| ||
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Roy (Verified Customer) (06-13-2024) | Works well at 1/1000 at 4°C overnight to detect CNOT9 by WB. (also validated through siRNA-mediated KD of CNOT9)
|
FH Abe (Verified Customer) (04-23-2024) | Worked well at 1:100 overnight at 4c.
|
