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CoraLite® Plus 488-conjugated CNBP Monoclonal antibody

CNBP Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H1D12

Cat no : CL488-67109

Synonyms

PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein, CCHC-type zinc finger nucleic acid binding protein

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CNBP Monoclonal anti (CL488-67109)

    FC experiment of HeLa using CL488-67109

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CNBP (CL488-67109, Clone:1H1D12) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67109 targets CNBP in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CNBP fusion protein Ag7114
Full Name CCHC-type zinc finger, nucleic acid binding protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19-25 kDa
GenBank Accession NumberBC000288
Gene Symbol CNBP
Gene ID (NCBI) 7555
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CNBP antibody CL488-67109Download protocol
Standard Protocols
Click here to view our Standard Protocols