CLUH Recombinant antibody

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  IF Staining of HeLa using 84321-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLUH antibody (84321-4-RR, Clone: 241640C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84321-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84321-4-RR against Human CLUH were performed. The affinity constant is 7.19 nM.

CLUH {ptg:Brand} Recombinant Antibody for IF/ICC, ELISA

Cat No. 84321-4-RR

Clone No.241640C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA0664, Protein KIAA0664, FLJ23672, CLU1, 241640C11

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84321-4-RR targets CLUH in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLUH fusion protein Ag34275
• Full Name: KIAA0664
• GenBank Accession Number: BC156940
• Gene Symbol: CLUH
• Gene ID (NCBI): 23277
• RRID: AB_3671863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75153
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)