CLUH Polyclonal antibody, PBS Only

• 

  WB analysis using 30986-1-AP (same clone as 30986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30986-1-AP (CLUH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30986-1-PBS in a different storage buffer formulation.

• 

  IF Staining of COS-7 using 30986-1-AP (same clone as 30986-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using CLUH antibody (30986-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30986-1-PBS in a different storage buffer formulation.

CLUH Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 30986-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0664, Protein KIAA0664, FLJ23672, CLU1

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Product Information

30986-1-PBS targets CLUH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLUH fusion protein Ag34275
• Full Name: KIAA0664
• Observed Molecular Weight: 147-150 kDa
• GenBank Accession Number: BC156940
• Gene Symbol: CLUH
• Gene ID (NCBI): 23277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O75153
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)