CLUH Polyclonal antibody

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  WB analysis using 30986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30986-1-AP (CLUH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of COS-7 using 30986-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using CLUH antibody (30986-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CLUH Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30986-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0664, Protein KIAA0664, FLJ23672, CLU1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, mouse kidney tissue
• Positive IF/ICC detected in: COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30986-1-AP targets CLUH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLUH fusion protein Ag34275
• Full Name: KIAA0664
• Observed Molecular Weight: 147-150 kDa
• GenBank Accession Number: BC156940
• Gene Symbol: CLUH
• Gene ID (NCBI): 23277
• RRID: AB_3669806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O75153
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)