CLTCL1 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66274-1-Ig (same clone as 66274-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66274-1-Ig (same clone as 66274-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66274-1-Ig (same clone as 66274-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66274-1-Ig (same clone as 66274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66274-1-Ig (same clone as 66274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66274-1-Ig (same clone as 66274-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

CLTCL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66274-1-PBS

Clone No.1B5A10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD

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Product Information

66274-1-PBS targets CLTCL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: clathrin, heavy chain-like 1
• Calculated Molecular Weight: 187 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_007098
• Gene Symbol: CLTCL1
• Gene ID (NCBI): 8218
• RRID: AB_2881657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53675
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.