CLPS Recombinant antibody

CLPS Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241565H3

Cat no : 84224-2-RR

Synonyms

Colipase, 241565H3

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Validation Data Gallery

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  WB analysis of rat pancreas using 84224-2-RR

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 84224-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 84224-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84224-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: rat pancreas tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84224-2-RR is CLPS Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLPS fusion protein Ag2321
• Full Name: colipase, pancreatic
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC017897
• Gene Symbol: CLPS
• Gene ID (NCBI): 1208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.