CLIC4 Monoclonal antibody

• Featured Product
• KD/KO Validated

CLIC4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B7F9

Cat no : 66343-1-Ig

Synonyms

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

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Validation Data Gallery

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  WB analysis of HeLa using 66343-1-Ig

  WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

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  WB analysis using 66343-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 66343-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HuH-7 cells, pig heart tissue, HeLa cells, U2Os cells, pig kidney tissue, rabbit kidney tissue, rat kidney tissue, mouse kidney tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66343-1-Ig targets CLIC4 in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CLIC4 fusion protein Ag2942
• Full Name: chloride intracellular channel 4
• Calculated Molecular Weight: 253 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC012444
• Gene Symbol: CLIC4
• Gene ID (NCBI): 25932
• RRID: AB_2881723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

Publications

Species	Application	Title
rat	WB,IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li