Anti-Human CLEC12A Rabbit Recombinant Antibody, PBS Only

CLEC12A Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240791B7

Cat no : 98083-1-PBS

Synonyms

CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98083-1-RR (same clone as 98083-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7), or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98083-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98083-1-RR (same clone as 98083-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7)(red), or 0.25 ug Isotype Control(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining.Cells were not fixed. This data was developed using the same antibody clone with 98083-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98083-1-RR (same clone as 98083-1-PBS)

  1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (CL647-65612, Clone: 3G8), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98083-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98083-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98083-1-RR against Human CLEC12A were performed. The affinity constant is 0.524 nM.

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Product Information

The immunogen of 98083-1-PBS is CLEC12A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: C-type lectin domain family 12, member A
• Calculated Molecular Weight: 31 kDa
• GenBank Accession Number: BC126291
• Gene Symbol: CLEC12A
• Gene ID (NCBI): 160364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).