CISD2-Specific Monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 66082-1-Ig (same clone as 66082-1-PBS)

  WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66082-1-Ig (same clone as 66082-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66082-1-Ig (same clone as 66082-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66082-1-Ig (same clone as 66082-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 66082-1-Ig (same clone as 66082-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66082-1-Ig (same clone as 66082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66082-1-Ig (same clone as 66082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CISD2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66082-1-PBS

Clone No.3D7A3

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

human, zebrafish, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

---

Product Information

66082-1-PBS targets CISD2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, zebrafish, rat, mouse samples.

• Tested Reactivity: human, zebrafish, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CDGSH iron sulfur domain 2
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: NM_001008388
• Gene Symbol: CISD2
• Gene ID (NCBI): 493856
• RRID: AB_11232425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N5K1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Front Pharmacol; Inhibition of CISD2 enhances sensitivity to doxorubicin in diffuse large B-cell lymphoma by regulating ferroptosis and ferritinophagy; Authors - Chaofeng Zhang