CISD2-Specific Monoclonal antibody

• Featured Product
• KD/KO Validated

CISD2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, zebrafish, rat, mouse

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3D7A3

Cat no : 66082-1-Ig

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

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Validation Data Gallery

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  WB analysis of HeLa using 66082-1-Ig

  WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells.

• 

  WB analysis of fetal human brain using 66082-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66082-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66082-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66082-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 66082-1-Ig

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 66082-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, MCF-7 cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, zebrafish tissue
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 66082-1-Ig is CISD2-Specific Fusion Protein expressed in E. coli.

• Tested Reactivity: human, zebrafish, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CDGSH iron sulfur domain 2
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: NM_001008388
• Gene Symbol: CISD2
• Gene ID (NCBI): 493856
• RRID: AB_11232425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.

Publications

Species	Application	Title
human	WB	Commun Biol; Integrating apaQTL and eQTL analysis identifies a potential causal variant associated with lung adenocarcinoma risk in the Chinese population; Authors - Huiwen Xu
human	WB	J Biomed Sci; Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis; Authors - Yu-Wen Hung
		Mol Neurodegener; Mitochondrial CISD1/Cisd accumulation blocks mitophagy and genetic or pharmacological inhibition rescues neurodegenerative phenotypes in Pink1/parkin models; Authors - Aitor Martinez
human,mouse	WB,CoIP	Nat Commun; ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome; Authors - Mailis Liiv