CIRBP Polyclonal antibody

• Featured Product
• KD/KO Validated

CIRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

80

Cat no : 10209-2-AP

Synonyms

CIRP, A18HNRNP, A18 hnRNP

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Validation Data Gallery

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  WB analysis of A549 cells using 10209-2-AP

  WB result of CIRBP antibody (10209-2-AP, 1:500) with si-control and si-CIRBP transfected A549 cell.

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  WB analysis using 10209-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 10209-2-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10209-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 10209-2-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10209-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10209-2-AP

  IP result of anti-CIRBP (IP:10209-2-AP, 3ug; Detection:10209-2-AP 1:500) with HepG2 cells lysate 6000ug.

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  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10209-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CIRBP antibody (10209-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of A549 using 10209-2-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CIRBP (10209-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: Y79 cells, A549 cells, HEK-293 cells, HepG2 cells, PC-12 cells, PC-13 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 20 publications below
• WB: See 66 publications below
• IHC: See 14 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

10209-2-AP targets CIRBP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, squirrel
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIRBP fusion protein Ag0280
• Full Name: cold inducible RNA binding protein
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC000403
• Gene Symbol: CIRBP
• Gene ID (NCBI): 1153
• RRID: AB_2080263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

Publications

Species	Application	Title
mouse	WB	Nature; RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.; Authors - Diego Peretti
human,squirrel	WB	Cell; iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.; Authors - Jingxing Ou
rat	WB	Nat Med; Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.; Authors - Qiang Xiaoling X
human	WB	Mol Cell; Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.; Authors - Nandan S Gokhale
human	WB	Nucleic Acids Res; Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection; Authors - Morgane Corre
human	WB,IHC	J Exp Clin Cancer Res; Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression; Authors - Lixia Li ; KD Validated

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