CoraLite® Plus 488-conjugated CIN85 Monoclonal antibody

CIN85 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C12D2

Cat no : CL488-66571

Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

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Validation Data Gallery

• 

  WB analysis using CL488-66571

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66571 (CIN85 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, THP-1 cells, HL-60 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66571 targets CIN85 in WB applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CIN85 fusion protein Ag2777
• Full Name: SH3-domain kinase binding protein 1
• Calculated Molecular Weight: 665 aa, 73 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC015806
• Gene Symbol: SH3KBP1/CIN85
• Gene ID (NCBI): 30011
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.