CoraLite® Plus 488-conjugated CHMP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHMP7 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16424

Synonyms

Chromatin-modifying protein 7, Chromatin modifying protein 7

---

Validation Data Gallery

• 

  IF Staining of A431 using CL488-16424

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CHMP7 antibody (CL488-16424) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16424 targets CHMP7 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHMP7 fusion protein Ag9625
• Full Name: CHMP family, member 7
• Calculated Molecular Weight: 453 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC019110
• Gene Symbol: CHMP7
• Gene ID (NCBI): 91782
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHMP7 (Charged multivesicular body protein 7), it is expected to be located in the nucleus and cytoplasm, which is ubiquitinated in lymph node and appendix. It is reported that Lem2p (LEM2), a member of the LEM (Lap2- Emerin-Man1) family of inner nuclear membrane proteins, and the ESCRT-II/ESCRT- III hybrid protein Cmp7p (CHMP7), work together to recruit additional ESCRT-III proteins to holes in the nuclear membrane. CHMP7 protein is involved in several processes, including late endosome to vacuole transport; midbody abscission; and mitotic nuclear division. It is a part of ESCRT III complex, which colocalizes with chromatin. The molecular weight of CHMP7 is 50 kDa.