CHMP2B Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12527-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 12527-1-AP (same clone as 12527-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 12527-1-AP (same clone as 12527-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 12527-1-AP (same clone as 12527-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12527-1-AP (same clone as 12527-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12527-1-AP (same clone as 12527-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12527-1-AP (same clone as 12527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PFA fixed cells using 12527-1-AP (same clone as 12527-1-PBS)

  IF result (endosomal stain) of anti-CHMP2B (12527-1-AP) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

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Product Information

12527-1-PBS targets CHMP2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHMP2B fusion protein Ag3222
• Full Name: chromatin modifying protein 2B
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC001553
• Gene Symbol: CHMP2B
• Gene ID (NCBI): 25978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.