CHCHD3 Recombinant antibody

• Featured Product
• KD/KO Validated

CHCHD3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4B11

Cat no : 82743-1-RR

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

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Validation Data Gallery

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  WB analysis using 82743-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82743-1-RR

  WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells.

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  IF Staining of HepG2 using 82743-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 82743-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82743-1-RR targets CHCHD3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHCHD3 fusion protein Ag22579
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC014839
• Gene Symbol: CHCHD3
• Gene ID (NCBI): 54927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.