CHCHD3 Recombinant antibody, PBS Only

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  WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

  WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 82743-1-RR (same clone as 82743-1-PBS)

  IP result of anti-CHCHD3 (IP:82743-1-RR, 4ug; Detection:82743-1-RR 1:1000) with Jurkat cells lysate 1600 ug. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82743-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

• Featured Product
• KD/KO Validated

CHCHD3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 82743-1-PBS

Clone No.4B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

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Product Information

82743-1-PBS targets CHCHD3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHCHD3 fusion protein Ag22579
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC014839
• Gene Symbol: CHCHD3
• Gene ID (NCBI): 54927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NX63
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.