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CHCHD3 Monoclonal antibody, PBS Only

CHCHD3 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8H12

Cat no : 68259-1-PBS

Synonyms

CHCHD3, FLJ20420

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD3 Monoclonal antibody (68259-1-Ig)

    WB analysis using 68259-1-Ig (same clone as 68259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CHCHD3 Monoclonal antibody (68259-1-Ig)

    WB analysis using 68259-1-Ig (same clone as 68259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CHCHD3 Monoclonal antibody (68259-1-Ig)

    WB analysis of HeLa using 68259-1-Ig (same clone as 68259-1-PBS)

    WB result of CHCHD3 antibody (68259-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HeLa cells. This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IF Staining of MCF-7 using 68259-1-Ig (same clone as 68259-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CHCHD3 antibody (68259-1-Ig, Clone: 1C8H12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68259-1-PBS targets CHCHD3 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHCHD3 fusion protein Ag22628
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC014839
Gene Symbol CHCHD3
Gene ID (NCBI) 54927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.