CHCHD3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CHCHD3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8H12

Cat no : 68259-1-PBS

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

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Validation Data Gallery

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  WB analysis using 68259-1-Ig (same clone as 68259-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  WB analysis using 68259-1-Ig (same clone as 68259-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68259-1-Ig (same clone as 68259-1-PBS)

  WB result of CHCHD3 antibody (68259-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HeLa cells. This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68259-1-Ig (same clone as 68259-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CHCHD3 antibody (68259-1-Ig, Clone: 1C8H12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68259-1-PBS in a different storage buffer formulation.

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Product Information

68259-1-PBS targets CHCHD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHCHD3 fusion protein Ag22628
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC014839
• Gene Symbol: CHCHD3
• Gene ID (NCBI): 54927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.