CHCHD3 Monoclonal antibody

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• KD/KO Validated

CHCHD3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C8H12

Cat no : 68259-1-Ig

Synonyms

CHCHD3, FLJ20420

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Validation Data Gallery

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  WB analysis using 68259-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68259-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68259-1-Ig

  WB result of CHCHD3 antibody (68259-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HeLa cells.

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  IF Staining of MCF-7 using 68259-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CHCHD3 antibody (68259-1-Ig, Clone: 1C8H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig heart tissue, A549 cells, HeLa cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat brain tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68259-1-Ig targets CHCHD3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHCHD3 fusion protein Ag22628
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC014839
• Gene Symbol: CHCHD3
• Gene ID (NCBI): 54927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

Publications

Species	Application	Title
rat	WB	Ann Transl Med; Electroacupuncture ameliorates surgery-induced spatial memory deficits by promoting mitophagy in rats; Authors - Jian Guo