CoraLite®594-conjugated CHCHD2 Monoclonal antibody

• Featured Product
• KD/KO Validated

CHCHD2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

2G1G10

Cat no : CL594-66302

Synonyms

C7orf17, CHCHD2, NS2TP

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66302

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66302 (CHCHD2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-66302 targets CHCHD2 in IF/ICC applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHCHD2 fusion protein Ag24219
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 2
• Calculated Molecular Weight: 151 aa, 16 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC003079
• Gene Symbol: CHCHD2
• Gene ID (NCBI): 51142
• RRID: AB_2883552
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHCHD2 is a widely expressed 17 kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. The function of the CHCH domain is not well understood, and the few characterized proteins that harbor this domain have diverse functions. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD).

Publications

Species	Application	Title
human	IF	iScience; Lipopolysaccharide induces placental mitochondrial dysfunction in murine and human systems by reducing MNRR1 levels via a TLR4-independent pathway; Authors - Neeraja Purandare