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  • Featured Product
  • KD/KO Validated

CHCHD2 Recombinant antibody, PBS Only

CHCHD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A12

Cat no : 82702-1-PBS

Synonyms

HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD2 Recombinant antibody (82702-1-RR)

    WB analysis using 82702-1-RR (same clone as 82702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82702-1-RR (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHCHD2 Recombinant antibody (82702-1-RR)

    WB analysis of HEK-293 using 82702-1-RR (same clone as 82702-1-PBS)

    WB result of CHCHD2 antibody (82702-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 82702-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD2 Recombinant antibody (82702-1-RR)

    IF Staining of HepG2 using 82702-1-RR (same clone as 82702-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (82702-1-RR, Clone: 4A12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82702-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82702-1-RR

    Affinity and Kinetic Characterization of 82702-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82702-1-RR against Human CHCHD2 were performed. The affinity constant is 2.12 nM.

Product Information

82702-1-PBS targets CHCHD2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHCHD2 fusion protein Ag13752
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Calculated Molecular Weight 151 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003079
Gene Symbol CHCHD2
Gene ID (NCBI) 51142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).