CHCHD10 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHCHD10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 25671-1-AP

Synonyms

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, Protein N27C7-4, Protein N27C7 4, N27C7 4, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial

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Validation Data Gallery

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  WB analysis of HEK-293 using 25671-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 25671-1-AP

  HAP1 (WT and CHCHD10 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 25671-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 25671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25671-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HAP1 using 25671-1-AP

  HAP1 lysates prepared and IP of CHCHD10 performed using 1.0 μg of 25671-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of mouse heart using 25671-1-AP

  IP result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:25671-1-AP 1:600) with mouse heart tissue lysate 4000ug.

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  IHC staining of human kidney using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25671-1-AP (CHCHD10 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25671-1-AP (CHCHD10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 25671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 25671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HAP1 using 25671-1-AP

  HAP1 WT cells (yellow outline) and CHCHD10 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 25671-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HAP1, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue, HAP1 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HAP1

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Product Information

25671-1-AP targets CHCHD10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD10 fusion protein Ag22598
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 10
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC065232
• Gene Symbol: CHCHD10
• Gene ID (NCBI): 400916
• RRID: AB_2880187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.; Authors - Jung-A A Woo ; KD Validated
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
mouse	IHC	J Mol Cell Biol; Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.; Authors - Jian Peng
mouse	WB	Diabetes; CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis; Authors - Meng Ding ; KO Validated
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
human	WB,IF	Hum Mol Genet; Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.; Authors - Isabella R Straub ; KD Validated