CHAT Monoclonal antibody, PBS Only

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  WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66816-1-Ig (same clone as 66816-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

CHAT Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66816-1-PBS

Clone No.1F7A9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase

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Product Information

66816-1-PBS targets CHAT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHAT fusion protein Ag19602
• Full Name: choline acetyltransferase
• Calculated Molecular Weight: 748 aa, 83 kDa
• Observed Molecular Weight: 67-71 kDa
• GenBank Accession Number: BC130617
• Gene Symbol: CHAT
• Gene ID (NCBI): 1103
• RRID: AB_2882159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28329
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.