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  • Western Blot (WB) analysis of various lysates using CHAT Monoclonal antibody (66816-1-Ig)

    WB analysis using 66816-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using CHAT Monoclonal antibody (66816-1-Ig)

    WB analysis of PC-12 using 66816-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of mouse small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of mouse small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of rat small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of rat small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHAT Monoclonal antibody

CHAT Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66816-1-Ig
Clone No.1F7A9

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inJurkat cells, PC-12 cells, HEK-293 cells, SH-SY5Y cells, Y79 cells, U-251 cells, Neuro-2a cells
Positive IHC detected inmouse small intestine tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66816-1-Ig targets CHAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CHAT fusion protein Ag19602 Predict reactive species
Full Name choline acetyltransferase
Calculated Molecular Weight 748 aa, 83 kDa
Observed Molecular Weight 67-71 kDa
GenBank Accession NumberBC130617
Gene Symbol CHAT
Gene ID (NCBI) 1103
RRIDAB_2882159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.

Protocols

Product Specific Protocols
WB protocol for CHAT antibody 66816-1-IgDownload protocol
IHC protocol for CHAT antibody 66816-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols