CoraLite® Plus 488-conjugated CEP63 Monoclonal antibody

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  IF Staining of hTERT-RPE1 using CL488-66996

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 CEP63 antibody (CL488-66996, Clone: 2G12B2 ) at dilution of 1:200.

CEP63 Monoclonal Antibody for IF/ICC

Cat No. CL488-66996

Clone No.2G12B2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66996 targets CEP63 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CEP63 fusion protein Ag9227
• Full Name: centrosomal protein 63kDa
• Calculated Molecular Weight: 703 aa, 81 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC014050
• Gene Symbol: CEP63
• Gene ID (NCBI): 80254
• RRID: AB_2934509
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96MT8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.