CEP120 Monoclonal antibody

• 

  WB analysis using 60468-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60468-1-Ig (CEP120 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CEP120 Monoclonal Antibody for WB, ELISA

Cat No. 60468-1-Ig

Clone No.1G12B4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

FLJ36090, Coiled-coil domain-containing protein 100, Centrosomal protein of 120 kDa, centrosomal protein 120kDa, CCDC100

---

Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, IMR-32 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60468-1-Ig targets CEP120 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CEP120 fusion protein Ag33947
• Full Name: centrosomal protein 120kDa
• Calculated Molecular Weight: 986 aa, 113 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC040527
• Gene Symbol: CEP120
• Gene ID (NCBI): 153241
• RRID: AB_3670261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N960
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP120 encodes a protein that functions in the microtubule-dependent coupling of the nucleus and the centrosome. It is involved in regulating centrosome-mediated interkinetic nuclear migration of neural progenitors (PMID: 27185865, 23857771). CEP120, SPICE1, and CPAP are required for centriole elongation and for the recruitment of distal microtubule-capping proteins and CEP135 to procentrioles (PMID: 23810536).